Peripheral nerve fibres form multifacet interactions with keratinocytes in a novel complete human 2D culture model

In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold

Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

Tapering of human nerve fibres.

To determine the tapering of human nerve fibres, rostral and caudal root pieces of cauda equina nerve roots were removed and nerve fibre diameter distributions were constructed for 4 myelin sheath thickness ranges for the two sites, and compared with each other. The reduction of the group diameter in the different alpha-motoneuron groups was 0.2 % per 13 cm. Accounting for systematic errors, th...

The distribution of electron-dense tracers in peripheral nerve fibres.

Two electron-dense tracers, ferritin and lanthanum, have been administered to peripheral nerve fibres, and their uptake has been studied ultrastructurally. It was found that the perineurium was an effective barrier to ferritin in vivo, and the tracer was subsequently injected sub-perineurially. Ferritin uptake over a 120-min period was confined to occasional phagocytic vesicles in perineurial a...

Aberrant Peripheral Nerve Fibres in the Medulla Oblongata of Man.

Refractory period of human motor nerve fibres.

Using a collision technique, the ulnar nerve was made refractory for a shorter distance normally covered in 0.5 ms or a longer distance covered in 1.5 ms. Studying the shorter refractory segment with paired shocks of maximal intensity, the test response first appeared (more than 5% of unconditioned response) at an interstimulus interval of 1.16 +/- 0.18 ms (mean +/- SD in 20 ulnar nerves). The ...